International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

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Original Article


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449- 452


Authors Details

Udaya Kumari Ganapathy, Nagendran Ramachandran


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D, L Glyceraldehyde- Will it be an effective Antiglycolytic Agent?


Original Article

Author Details : Udaya Kumari Ganapathy, Nagendran Ramachandran

Volume : 3, Issue : 4, Year : 2016

Article Page : 449-452


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Abstract

Aim: This study aimed to compare the effectiveness of D, L Glyceraldehyde (GLY) with Plain Sodium Fluoride (NaF) as an antiglycolytic agent and its interferences in the estimation of Serum creatinine by Modified Jaffe?s method and Serum Sodium and Potassium by ion selective electrode(ISE) method.
Materials and Methods: Eighty random blood samples were collected from the OP patient after obtaining explicit consent. Serum Glucose, Creatinine, Sodium and Potassium were estimated in all the three tubes and the results were compared across.
Statistical Analysis: ANOVA test was done to compare the analytical values obtained from all the three tubes.
Results: Mean Glucose concentration in Plain Tube with Clot activator (PTC), NaF tube and the GLY tube were 142.25 mg/dl, 124.41mg/dl and 139.23mg/dl respectively. p-value for GLY tube vs NaF was 0.483, GLY tube vs PTC was 0.970 and NaF vs PTC was 0.349(p value < 0.05 considered significant). There was Positive interference with creatinine measurement by modified Jaffe’s method (p<0.0001) and for Serum Potassium estimation by ISE method (p<0.0001). Both sodium and potassium estimation in NaF tube showed significantly increased values when compared to analysis in PTC.
Conclusions: For preventing ex vivo glycolysis, D, L-Glyceraldehyde coated tubes is as good as PTC tube for glucose estimation by glucose oxidase peroxidase (GOD-POD) method. D, L Glyceraldehyde coated tubes can be used for the estimation of serum sodium but not for potassium by ISE method and creatinine by Modified Jaffe’s method. PTC tube cannot be replaced by GLY tube for routine chemistry analysis.

Keywords: D, L Glyceraldehyde, Plain tube with clot activator, Sodium Fluoride, Antiglycolytic agent, Glycolysis, Glucose


How to cite : Ganapathy U K, Ramachandran N, D, L Glyceraldehyde- Will it be an effective Antiglycolytic Agent?. Int J Clin Biochem Res 2016;3(4):449-452

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