International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

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Original Article


Article page

301- 305


Authors Details

Tanu Verma*, Rohit Lal, Manish Kumar, Uday Kumar, S. K. Shahi


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Association of Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in breast cancer patients of Bihar: Case-control study


Original Article

Author Details : Tanu Verma*, Rohit Lal, Manish Kumar, Uday Kumar, S. K. Shahi

Volume : 9, Issue : 4, Year : 2022

Article Page : 301-305

https://doi.org/10.18231/j.ijcbr.2022.059



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Abstract

Background: Breast cancer (BC) is the most frequent cancer in women globally and the main cause of cancer-related mortality. MTHFR C677T is a functional polymorphism in the genes encoding a one-carbon metabolism enzyme that affect folate metabolism and may be associated with breast cancer susceptibility.
Method: We have conducted a case-control study taking 100 cases of diagnosed breast cancer and 100 healthy control. PCR-RFLP technique was used to detect the genotype of C677T polymorphism in the study population.
Result: About 35% of cases had normal genotype(C677C), 49% had the genotype C677T & rest 16% had the genotype T677T for MTHFR. Among control the frequency of C677C genotype was 55% C677T was 40% & T677T was 5%. C677T & T677T MTHFR genotype were more common in case as compared to control [?2 (2, N = 200) = 11.12, p = 0.004]. T allele frequency was significantly higher in case (40%) as compared to control (5%) [?2 (1, N = 400) = 10.91, p = 0.001]. Cases with MTHFR 677TT genotype had a significantly higher risk of BC than 677CC individual [OR=1.64 (1.03 to 2.10)] and increasing T-allele was significant in BC [OR=1.07 (0.71 to 1.33)] (P for trend = 0.04).
Conclusion: C677T of MTHFR gene has a strong correlation with breast cancer in the studied population and can be used as a prognostic marker for the development of breast cancer.
 

Keywords: Methylenetetrahydrofolate reductase (MTHFR), Polymorphism, PCR­RFLP, Folate.


How to cite : Verma T, Lal R, Kumar M, Kumar U, Shahi S K, Association of Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in breast cancer patients of Bihar: Case-control study. Int J Clin Biochem Res 2022;9(4):301-305

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