International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377


International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the  Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing more...

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Dhiraj J Trivedi*

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Detection of Alkaptonuria by simple, effective and precise chemical methods: A technical review

Review Article

Author Details : Dhiraj J Trivedi*

Volume : 7, Issue : 3, Year : 2020

Article Page : 297-301

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Alkaptonuria is a rare error in tyrosine metabolism. Due to genetic variants severity of disease vary.
Lack of local expertise and difficulty in detection of AKU has been expressed in literature. Onset of
clinical symptoms and ochronosis starts before adult age but timely detection at paediatric age is useful
in prevention of ochronotic arthropathy.
Objective of this technical review is to provide simple, quick and precise chemical methods with reagent
and technical details so as to detect HGA in urine at every primary health care setups to pick up AKU at
early age.
Conclusion: Present technical review explains eight precise, time tested, simple and effective chemical
methods. They are technically less demanding and can be used at primary centre to even at bed side
clinics. Review may increase screening of the disease by medical practitioners and may encourage budding
researchers to contribute in creating data base for prevalence of AKU in India.

Keywords: Alkaptonuria, Homogentisic acid, Ochronosis, Rare disease, Homogentisate dioxygenase, Chemical reactions.

How to cite : Trivedi D J , Detection of Alkaptonuria by simple, effective and precise chemical methods: A technical review. Int J Clin Biochem Res 2020;7(3):297-301

Copyright © 2020 by author(s) and Int J Clin Biochem Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (

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